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Capricorn Scientific成立于2013年，是一家新的德國細胞培養基，血清和試劑生產商。我們生產的主要重點是生產用于生物技術，診斷和研究應用的高質量動物血清。嚴格的測試程序以及全面的可追溯性記錄和文檔保證了我們產品的和可重復的質量。
作為一家規模較小的公司，我們可以靈活地提供各種傳統和定制產品，以更低的成本，更短的交付周期和個性化的客戶服務滿足當今市場不斷增長的需求。
我們的技術，經驗豐富的專家團隊多年來一直致力于細胞培養領域。我們致力于為活躍的細胞培養公司提供高質量，可靠性和服務。
Capricorn Scientific Hy-A說明書
HybridomaPlus Protein-free Medium for Hybridoma Cells and Monoclonal Antibody Production with L-Glutamine
雜交瘤細胞無蛋白培養基及L-谷氨酰胺制備單克隆抗體的研究
貨號：Hy-A(500毫升)
產品闡述，產品說明
雜交瘤加是一種無蛋白質的、可隨時使用的培養基，不含多肽生長或附著因子，或可能使下游加工和終產品純化復雜化的中介物。H型 此外，YbridomaPlus還能很好地用于制備單克隆抗體的血清培養基，也可作為一種生長介質。
商品概述
pH 7.0 – 7.5
內毒素≤1 EU/ml
細胞培養試驗
不育試驗
儲存2°C至8°C
重要信息
-雜交瘤加需要補充膽固醇補充劑或其他來源的膽固醇，以生長依賴膽固醇的細胞系(例如NS0和衍生物)。
-雜交瘤加不含胰島素或轉鐵蛋白。
-這種介質不含表面活性劑。如果用于攪拌懸浮培養，添加0.1%Pluronic F-68。
-在大多數情況下，抗生素是不必要的，也不是建議的。然而，在需要抗生素的地方，大多數普通抗生素都與雜交瘤加兼容，包括青霉素/鏈霉菌。 在，慶大霉素，抗PPLO，亞諾辛，兩性霉素B。不要使用硫酸卡那霉素或新霉素硫酸鹽。
培養條件
文化類型：暫停
培養容器：搖瓶、滾筒瓶或生物反應器。
溫度范圍：36℃至38℃
孵化器氣氛：空氣中5%至10%CO2的加濕空氣。確保適當的氣體交換，盡量減少文化暴露在光線下。
恢復，復原
在37°C水浴中快速解凍(<1分鐘)冷凍細胞。
將冷凍瓶的全部內容轉移到不含抗生素的30毫升預溫雜交瘤加的組織培養瓶中。
37°C在空氣中5%CO2的加濕環境中孵育。
4.解凍后3~5天傳代細胞。
使雜交瘤細胞適應雜交瘤+
成功的適應將取決于特定的雜交瘤細胞株和所使用的培養條件。我們建議將原始培養基中的備份培養保持到成功為止。 新的媒介已經實現。
注：細胞存活率至少為90%，細胞在適應前處于對數中期生長階段，這是至關重要的。
直接適應，直接適應作用
傳代雜交瘤細胞在常規培養基中加入5%~10%血清或其他無血清培養基培養成預溫雜交瘤細胞。在適應過程中，播種密度應加倍。 細胞株的正常播種密度。
監測細胞生長，直到活細胞密度達到1×106個活細胞/ml。將細胞傳代至1~2×105個活細胞密度為1~2×105個/ml。
繼續監測和傳代細胞3至5代，直到達到一致的生長。
注：如果使用直接適應法在3~5通道上觀察到次優性能，則使用順序適應法。
順序適應
傳代雜交瘤細胞在常規培養基中加入5%~10%的血清或其他無血清培養基培養成25：75的新鮮雜交瘤細胞，與原培養基的比例為25：75。適應程序期間 種子密度為正常播種密度的兩倍。
監測細胞生長，直到活細胞密度達到1×106個活細胞/ml。繼代培養細胞(稀釋為1~2×105個活細胞/ml)，逐步增加新鮮雜交瘤細胞與Ori的比例。 繼代培養基(50：50，75：25，90：10，100雜交加)。每個步驟可能需要多個通道。
繼續監測和傳代細胞，直到細胞持續生長。在*雜交中進行了幾次連續生長和存活的傳代后，這種培養被認為是無性的。 [醫][=pulmonary thromboembolic disease]肺動脈血栓栓塞疾病.
冷凍保存，低溫貯藏
在組織培養瓶中準備所需數量的細胞，在生長中期收獲，活率>90%。保留條件培養基準備冷凍保存培養基。
測定活細胞密度，計算冷凍保存培養基所需體積，得出終細胞密度為0.5~1×107個/ml。
在預定使用日制備92.5%培養基(新鮮培養基與條件培養基的比例為50：50)7.5%DMSO的要求體積。過濾，消毒，4°C保存，直到使用 。重要的是：條件培養基應從高活力的中對數培養細胞中獲得.
100×g離心收集細胞5~10 min。將球團置于預先確定的4°C冷凍保存介質中。
根據制造商的規格將此懸浮液配發到冷凍瓶中(即2毫升冰瓶中的1毫升)。
按照標準程序(每分鐘減少1°C)，在自動化或手動控制的冷凍設備中實現低溫保存。
將冷凍細胞轉移到液氮(蒸汽相)，建議儲存在-200°C至-125°C。
預防措施及免責聲明
本產品僅供研究用。請查閱材料安全數據表，以獲取有關危險和安全處理做法的信息。
Pluronic是巴斯夫的注冊商標。
我們生產和測試了我們的生物加工和細胞培養血清產品，用于不同的哺乳動物細胞培養應用。我們提供各種各樣的特色血清：
液體培養基為所有細胞培養應用提供基本營養。我們的每種高質量細胞培養基都是根據初公布的配方或對培養基的一致性能和穩定性*的修飾而制造的。使用摩羯座培養基是保證細胞成功生長的宜方法。
我們提供各種產品：
來自摩羯座的試劑和補充劑允許您定制細胞培養系統的生長條件。所有產品都經過嚴格測試和設計，以提供可重復性和良性能。
我們提供各種各樣的產品：
Capricorn Scientific產品目錄：
| 貨號 | 品名 | 規格 | 價格 |
| DMEM-LPA | DMEM Low Glucose (1 g/l), with L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
| DMEM-LPSTA | DMEM Low Glucose (1 g/l), with Stable Glutamine, with Sodium Pyruvate | 500 ml | 9.50 € |
| DMEM-LPXA | DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
| DMEM-HA | DMEM High Glucose (4.5 g/l), with L-Glutamine | 500 ml | 6.40 € |
| DMEM-HHSTA | DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES | 500 ml | 9.50 € |
| DMEM-HPA | DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
| DMEM-HPSTA | DMEM High Glucose (4.5 g/l), with Stable Glutamine, with Sodium Pyruvate | 500 ml | 7.00 € |
| DMEM-HPXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate | 500 ml | 7.50 € |
| DMEM-HXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine | 500 ml | 6.40 € |
| DMEM-HXRXA | DMEM High Glucose (4.5 g/l), w/o L-Glutamine, w/o Phenol Red | 500 ml | 10.50 € |
| DMEM-12-A | DMEM/Ham’s F-12, with L-Glutamine | 500 ml | 8.60 € |
| DMEM-12-HXA | DMEM/Ham’s F-12, w/o L-Glutamine, with 15 mM HEPES | 500 ml | 8.60 € |
| HAM-12-A | Ham’s F-12, with L-Glutamine | 500 ml | 9.50 € |
| IMDM-A | IMDM, with L-Glutamine, without Supplements | 500 ml | 9.00 € |
| L15-XA | Leibovitz’s L-15 Medium, w/o L-Glutamine | 500 ml | 12.40 € |
| MCC-A | McCoy’s Medium, with L-Glutamine | 500 ml | 12.70 € |
| M199-A | Medium 199, with L-Glutamine | 500 ml | 8.90 € |
| MEM-A | MEM with Earle’s Salts, with L-Glutamine | 500 ml | 7.20 € |
| MEM-STA | MEM with Earle’s Salts, with Stable Glutamine | 500 ml | 9.40 € |
| MEM-XA | MEM with Earle’s Salts, w/o L-Glutamine | 500 ml | 6.40 € |
| MEMA-RXA | MEM Alpha Modification, w/o L-Glutamine, with Nucleosides | 500 ml | 19.90 € |
| MEMA-XRXA | MEM Alpha Modification, w/o L-Glutamine, w/o Nucleosides | 500 ml | 13.00 € |
| RPMI-A | RPMI 1640, with L-Glutamine | 500 ml | 6.40 € |
| RPMI-HA | RPMI 1640, with L-Glutamine, with 25 mM HEPES | 500 ml | 9.90 € |
| RPMI-HXA | RPMI 1640, w/o L-Glutamine, with 25 mM HEPES | 500 ml | 9.50 € |
| RPMI-STA | RPMI 1640, with Stable Glutamine | 500 ml | 8.40 € |
| RPMI-XA | RPMI 1640, w/o L-Glutamine | 500 ml | 5.80 € |
| RPMI-XRXA | RPMI 1640, w/o L-Glutamine, w/o Phenol Red | 500 ml | 9.90 € |
| FM1-F | FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum | 50 ml | 65.00 € |
| FM2-F | FreezeMe Two, Cryopreservation Medium Serum-free | 50 ml | 70.00 € |
| TP-A | TumorPlus 263, Complete Medium for Tumor Cells, with L-Glutamine | 500 ml | 29.90 € |
| HP-A | HybridomaPrime, Hybridoma Medium, Serum-free | 500 ml | 34.90 € |
| HY-A | HybridomaPlus, Hybridoma Medium, Protein-free | 500 ml | 39.90 € |
| FP-A | FibroPlus 333 Complete Medium for Fibroblasts, with L-Glutamine | 500 ml | 29.90 € |
| ERP-B | EndoPrime Complete Medium for C*tion of Primary Cells | 100 ml | on request |
| ERP-A | EndoPrime Complete Medium for C*tion of Primary Cells | 500 ml | on request |
| HPR-B | HepaPrime Complete Medium for C*tion of Primary Hepatocytes | 100 ml | on request |
| HPR-A | HepaPrime Complete Medium for C*tion of Primary Hepatocytes | 500 ml | on request |
| NKR-G | NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells | 20 ml | on request |
| NKR-B | NK-Prime Complete Medium for C*tion of Primary Natural Killer Cells | 100 ml | on request |
| CHOA-500ML | CHO-ONE A | 500 ml | 45.00 € |
| CHOE-500ML | CHO-ONE E | 500 ml | 49.00 € |
| CHOE-1000ML | CHO-ONE E | 1000ml | 89.00 € |
| CHOF1-100ML | CHO-ONE F1 | 100 ml | 23.00 € |
| CHOF1-500ML | CHO-ONE F1 | 500 ml | 95.00 € |
| CHOF2-10ML | CHO-ONE F2 | 10 ml | 15.00 € |
| CHOF2-50ML | CHO-ONE F2 | 50 ml | 49.00 € |
| CHO-K1 | CHO-ONE Starter Kit |
| 169.00 € |
| CHO-K2 | CHO-ONE Expansion Kit |
| 950.00 € |
| DMEM-LPA-P10 | DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 10 L | 14.90 € |
| DMEM-LPA-P50 | DMEM Low Glucose (1 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 50 L | 62.90 € |
| DMEM-HA-P10 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 15.50 € |
| DMEM-HA-P50 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 66.50 € |
| DMEM-HPA-P10 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 10 L | 15.50 € |
| DMEM-HPA-P50 | DMEM High Glucose (4.5 g/l) powder medium, with L-Glutamine, with Sodium Pyruvate, w/o Sodium Bicarbonate | 50 L | 66.50 € |
| MEM-A-P10 | MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 14.90 € |
| MEM-A-P50 | MEM with Earle’s Salts powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 62.90 € |
| RPMI-A-P10 | RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 10 L | 15.50 € |
| RPMI-A-P50 | RPMI 1640 powder medium, with L-Glutamine, w/o Sodium Bicarbonate | 50 L | 66.50 € |
| RPMI-HA-P10 | RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate | 10 L | 20.10 € |
| RPMI-HA-P50 | RPMI 1640 powder medium, with L-Glutamine, with 25 mM HEPES, w/o Sodium Bicarbonate | 50 L | 89.90 € |
| FBS-52B | Fetal Bovine Serum, Collected in Australia | 100 ml | on request |
| FBS-52A | Fetal Bovine Serum, Collected in Australia | 500 ml | on request |
| FBS-12B | Fetal Bovine Serum, Collected in South America | 100 ml | on request |
| FBS-12A | Fetal Bovine Serum, Collected in South America | 500 ml | on request |
| FBS-22B | Fetal Bovine Serum, Collected in USA | 100 ml | on request |
| FBS-22A | Fetal Bovine Serum, Collected in USA | 500 ml | on request |
| FBS-HI-12B | Fetal Bovine Serum, Heat Inactivated, Collected in South America | 100 ml | on request |
| FBS-HI-12A | Fetal Bovine Serum, Heat Inactivated, Collected in South America | 500 ml | on request |
| FBS-HI-22B | Fetal Bovine Serum, Heat Inactivated, Collected in USA | 100 ml | on request |
| FBS-HI-22A | Fetal Bovine Serum, Heat Inactivated, Collected in USA | 500 ml | on request |
| FBS-11B | Fetal Bovine Serum Advanced, Collected in South America | 100 ml | on request |
| FBS-11A | Fetal Bovine Serum Advanced, Collected in South America | 500 ml | on request |
| FBS-HI-11B | Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America | 100 ml | on request |
| FBS-HI-11A | Fetal Bovine Serum Advanced, Heat Inactivated, Collected in South America | 500 ml | on request |
| FBS-TET-12B | Fetal Bovine Serum, Tetracycline Negative, Collected in South America | 100 ml | on request |
| FBS-TET-12A | Fetal Bovine Serum, Tetracycline Negative, Collected in South America | 500 ml | on request |
| FBS-ES-22B | Fetal Bovine Serum, ES cell pre-tested, Collected in USA | 100 ml | on request |
| FBS-ES-22A | Fetal Bovine Serum, ES cell pre-tested, Collected in USA | 500 ml | on request |
| FBS-DIA-12B | Fetal Bovine Serum, Dialysed, Collected in South America | 100 ml | on request |
| FBS-DIA-12A | Fetal Bovine Serum, Dialysed, Collected in South America | 500 ml | on request |
| FBS-IG-12B | Fetal Bovine Serum, IgG Stripped, Collected in South America | 100 ml | on request |
| FBS-IG-12B | Fetal Bovine Serum, IgG Stripped, Collected in South America | 500 ml | on request |
| FBS-ED-12F | Fetal Bovine Serum Exosome Depleted | 50 ml | on request |
| FBS-ED-12B | Fetal Bovine Serum Exosome Depleted | 100 ml | on request |
| FBS-ED-12A | Fetal Bovine Serum Exosome Depleted | 500 ml | on request |
| NCS-1B | Newborn Calf Serum | 100 ml | 12.00 € |
| NCS-1A | Newborn Calf Serum | 500 ml | 38.00 € |
| NCS-HI-1B | Newborn Calf Serum, Heat Inactivated | 100 ml | 13.10 € |
| NCS-HI-1A | Newborn Calf Serum, Heat Inactivated | 500 ml | 43.00 € |
| CS-1B | Calf Serum | 100 ml | 8.00 € |
| CS-1A | Calf Serum | 500 ml | 29.50 € |
| ABS-1B | Adult Bovine Serum | 100 ml | 8.00 € |
| ABS-1A | Adult Bovine Serum | 500 ml | 29.50 € |
| ABS-1C | Adult Bovine Serum | 1000 ml | on reques |
| HOS-1B | Horse Serum | 100 ml | 7.20 € |
| HOS-1A | Horse Serum | 500 ml | 32.00 € |
| DHS-1B | Donor Horse Serum | 100 ml | 9.00 € |
| DHS-1A | Donor Horse Serum | 500 ml | 35.00 € |
| GOA-1B | Goat Serum | 100 ml | 24.00 € |
| GOA-1A | Goat Serum | 500 ml | 105.00 € |
| POR-1B | Porcine Serum | 100 ml | 10.50 € |
| POR-1A | Porcine Serum | 500 ml | 39.00 € |
| MOU-1B | Mouse Serum | 100 ml | 499.00 € |
| RAT-1B | Rat Serum | 100 ml | 499.00 € |
| RAB-1B | Rabbit Serum | 100 ml | 99.00 € |
| HUM-3B | Human Serum, Type AB, Male | 100 ml | 134.00 € |
| BSA-1U | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 50 g | 56.00 € |
| BSA-1S | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 100 g | 77.00 € |
| BSA-1V | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 250 g | on request |
| BSA-1T | Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA | 500 g | 299.00 € |
| BSA-FAF-1U | Bovine Serum Albumin, Fatty Acid Free, Origin USA | 50 g | 99.00 € |
| BSA-FAF-1S | Bovine Serum Albumin, Fatty Acid Free, Origin USA | 100 g | 178.00 € |
| BSA-PF-1U | Bovine Serum Albumin, Protease Free, Origin USA | 50 g | 88.00 € |
| BSA-PF-1S | Bovine Serum Albumin, Protease Free, Origin USA | 100 g | 151.00 € |
| HSA-1R | Human Serum Albumin | 10 g | 56.00 € |
| HSA-1U | Human Serum Albumin | 50 g | 227.00 € |
| HSA-1S | Human Serum Albumin | 100 g | 420.00 € |
| N2-K | N2 Supplement (100x), Serum-free | 5 ml | 45.00 € |
| C21-H | NCS21 Supplement (50x), Serum-free | 10 ml | 45.00 € |
| ITS-H | ITS (100x), Insulin, Transferrin, Selenium | 10 ml | 25.00 € |
| HCS-F | Hybridoma Supplément, Serum-free | 50 ml | 99.00 € |
| GLN-B | L-Glutamine, 200 mM | 100 ml | 8.00 € |
| STA-B | Stable Glutamine, 200 mM | 100 ml | 14.50 € |
| NPY-B | Sodium Pyruvate, 100 mM | 100 ml | 10.50 € |
| NEAA-B | MEM Nonessential Amino Acids (100x) | 100 ml | 8.50 € |
| ACC-1B | Accutase Cell Detachment Solution | 100 ml | 26.00 € |
| ACM-1G | AccuMax Cell Detachment Solution | 20 ml | 29.00 € |
| ACM-1F | AccuMax Cell Detachment Solution | 50 ml | 69.00 € |
| TRY-1B | Trypsin-EDTA (0.05 %) in DPBS (1x) | 100 ml | 7.00 € |
| TRY-1B10 | Trypsin-EDTA (0.5 %) in DPBS (10x) | 100 ml | 11.50 € |
| TRY-2B | Trypsin (0.25 %) in DPBS (1x) | 100 ml | 7.00 € |
| TRY-2B10 | Trypsin (2.5 %) in DPBS (10x) | 100 ml | 11.50 € |
| TRY-3B | Trypsin-EDTA (0.25 %) in HBSS (1x) with Phenol Red | 100 ml | 9.50 € |
| TRY-4B | Trypsin-EDTA (0.05 %) in HBSS (1x) with Phenol Red | 100 ml | 8.50 € |
| AMP-B | Amphotericin B Solution (250 µg/ml) | 100 ml | 14.00 € |
| AAS-B | Antibiotic/Antimycotic Solution (100x) | 100 ml | 14.00 € |
| GEN-10B | Gentamicin Solution (10 mg/ml) | 100 ml | 50.00 € |
| PS-B | Penicillin/Streptomycin (100x) | 100 ml | 8.00 € |
| PSG-B | Penicillin/Streptomycin (100x), with L-Glutamine | 100 ml | 15.00 € |
| MYX-B | MycoXpert, Mycoplasma Removal Reagent (50x) | 100 ml | 99.00 € |
| LSM-B | Lymphocytes Separation Media, Density 1.077 g/ml | 100 ml | 12.00 € |
| LSM-A | Lymphocytes Separation Media, Density 1.077 g/ml | 500 ml | 36.50 € |
| FM1-F | FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum | 50 ml | 65.00 € |
| FM2-F | FreezeMe Two, Cryopreservation Medium Serum-free | 50 ml | 70.00 € |
| MYX-B | MycoXpert, Mycoplasma Removal Reagent (50x) | 100 ml | 99.00 € |
| PBS-10XA | Dulbecco's PBS (10x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 13.00 € |
| PBS-1A | Dulbecco's PBS (1x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 6.50 € |
| PBS-2A | Dulbecco's PBS (1x), with Ca & Mg, w/o Phenol Red | 500 ml | 8.00 € |
| HBSS-1A | Hanks' Balanced Salts (1x), with Ca & Mg, w/o Phenol Red | 500 ml | 7.50 € |
| HBSS-2A | Hanks' Balanced Salts (1x), w/o Ca & Mg, w/o Phenol Red | 500 ml | 7.00 € |
| HBSS-3A | Hanks' Balanced Salts (1x), w/o Ca & Mg, with Phenol Red | 500 ml | 7.50 € |
| HEP-B | HEPES Buffer Solution (1 M) | 100 ml | 39.00 € |
| PBS-1A-P10 | Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red | 10 L | 10.90 € |
| PBS-1A-P50 | Dulbecco's PBS (1x) powder, w/o Ca & Mg, w/o Phenol Red | 50 L | 43.10 € |
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