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BioChain是高質量加工生物樣品產品和分析服務的先進者，用于推進轉化醫學，伴隨診斷和臨床產品研發。BioChain的產品可用于DNA和RNA測序，PCR和RT-PCR，基因表達分析，DNA和RNA純化，蛋白質提取和純化以及蛋白質表達分析。BioChain的下一代測序一站式服務，從臨床樣品采集，樣品制備，測序到生物信息學分析，將*改變行業。
除了我們*的生物標本和樣品制備業務，BioChain正在進入臨床應用領域，為癌癥和生殖健康提供臨床基因組學診斷產品和臨床試驗服務。BioChain致力于通過持續流程改進計劃以競爭力的價格實現高質量的產品和服務。BioChain建立了全面的質量保證計劃，以滿足客戶的滿意度，并為我們的內部生產設施保持ISO 9001：2015和ISO 13485：2003認證。
概觀
去除引物，酶，短DNA段，鹽和其他緩沖液成分可提高從PCR片段獲得的DNA序列數據的質量。BioChain的SeqPure提供了一種，方便且經濟的方法來清除長度超過100bp的DNA。
描述
SeqPure™PCR試劑是一種基于順磁珠技術的后PCR和下一代文庫制備清洗系統，旨在有效純化PCR擴增子。純化包括除去鹽，引物，引物二聚體，dNTP，因為DNA段選擇性地與磁珠顆粒結合; 用低鹽洗脫緩沖液或水洗脫高純度的DNA，可直接用于下游應用。該協議可以使用您當前的協議適應您當前的液體處理工作站（例如Beckman，Hamilton，Tecan，Caliper，Perkin Elmer，Agilent和Eppendorf），也可以手動執行。
•PCR•突變檢測和基因分型•測序（Sanger和下一代）•微陣列•限制酶清除•克隆•片段分析
•與主要競爭對手的性能相似，價格低廉•在半小時內凈化PCR產品•去除鹽，dNTP，引物和酶•無需離心或真空過濾•無花式緩沖液; 只需使用乙醇和Tris-HCI / TE•易于自動化•適用于96孔和384孔格式•可擴展：只使用盡可能多的珠子
用SeqPure珠和Ampure珠純化等量的含有鹽和蛋白質的100bp梯子。使用不同的緩沖液TE和預熱的TE（用于增加產率）進行洗脫。加載洗脫的DNA（各8μl）并在1％瓊脂糖凝膠上電泳。凝膠顯示出3000-100bp的所有片段的優異回收率。
SeqPure和AmPure在純化含有鹽和蛋白質的100 bp DNA梯中的比較。通過分光光度法測量純度。260/280和260/230吸光度比均表明SeqPure可有效去除鹽和蛋白質，與AmPure相當。
對于許多分子生物學技術，從DNA中去除污染物對下游應用至關重要。例如，為了確保成功的測序反應，必須從模板中除去所有過量的污染物，例如引物，短缺失的PCR產物，未摻入的dNTP，鹽和殘留的蛋白質。這些試劑可以干擾后續反應并導致不可讀的序列。雖然去除多余的污染物是產生高質量數據的關鍵，但這樣做可能非常耗時。PCR純化試劑盒可以方便地清除DNA，可以顯著提高實驗室的生產率。可以手動使用的小樣本套件，同時可以在各種平臺上輕松實現自動化，確保所有樣品通量都采用相同的程序 - 確保一致的性能。這樣的試劑盒也應該是有效的，并且以小的損失純化DNA段。后，它應該具有成本效益。 BioChain的SeqPure PCR純化試劑盒專為快速有效純化PCR產物而設計。該系統利用順磁珠技術選擇性地結合100bp和更大尺寸的DNA段。在洗脫純化的PCR產物之前，簡單的洗滌步驟將除去鹽和其他緩沖液成分，引物，引物二聚體，核苷酸和酶。SeqPure避免使用旋轉柱和其他純化膜，以便使試劑盒便于手動使用或自動化廣泛的實驗室自動化選擇（例如KingFisher，Beckman，Hamilton，Tecan，Caliper，Perkin Elmer，Agilent和Eppendorf）中。96-和384-井格式可以很容易地適應這種技術。用順磁顆粒而不是旋轉柱進行純化可以減少水動力剪切 - 提高較大DNA段的完整性。順磁珠技術的可擴展性也使該套件更加靈活。如果您正在凈化少量DNA，您可以使用更少的珠子，并為每個純化試劑盒提取更多樣品。
K5011610 | cfPure® Cell Free DNA Extraction Kit (100 ml) | 1 Kit (100 ml) | biochain |
K5011625 | cfPure® Cell Free DNA Extraction Kit (250 ml) | 1 Kit (250 ml) | biochain |
K5011625MA | cfPure® MAX Cell-Free DNA Extraction Kit | 1 Kit (250 ml) | biochain |
Z1510001 | SeqPure PCR Purification Kit (5 ml) | 5 ml | biochain |
Z1510002 | SeqPure PCR Purification Kit (50 ml) | 50 ml | biochain |
Z1510003 | SeqPure PCR Purification Kit (500 ml) | 500 ml | biochain |
L5011001 | PureSil-Silica Magnetic Beads | 1 L | biochain |
L5011010 | PureSil-Silica Magnetic Beads | 10 mL | biochain |
Z1322006 | AnaPrep Bacterial DNA Extraction Kit (48) For extracting genomic DNA from bacteria | 1 kit (48 extractions) | biochain |
Z1322002 | AnaPrep Blood DNA Extraction Kit 1200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 1 ml | 1 kit (48 extractions) | biochain |
Z1322001 | AnaPrep Blood DNA Extraction Kit 200 (48) For extracting genomic DNA from mammalian whole blood, peripheral mononuclear cell, or buffy coat. Sample volume range: up to 400 µl | 1 kit (48 extractions) | biochain |
Z1322005 | AnaPrep Cultured Cell DNA Extraction Kit (48) For extracting genomic DNA from up to 5×105 cultured cells | 1 kit (48 extractions) | biochain |
Z1322009 | AnaPrep FFPE DNA Extraction Kit (48) For extracting genomic DNA from formalin-fixed paraffin-embedded tissue (FFPE) samples | 1 kit (48 extractions) | biochain |
Z1322010 | AnaPrep Forensic DNA Extraction Kit (48) For extracting genomic DNA from a wide range of forensic and human identity samples | 1 kit (48 extractions) | biochain |
Z1322007 | AnaPrep HPV DNA Extraction Kit for swab samples (48) For extracting HPV DNA from swab samples | 1 kit (48 extractions) | biochain |
Z1322008 | AnaPrep TB DNA Extraction Kit (48) For extracting Mycobacterium tuberculosis DNA from sputum | 1 kit (48 extractions) | biochain |
Z1322004 | AnaPrep Tissue DNA Extraction Kit (48) For extracting genomic DNA from a variety of animal tissues, swab, and blood stains | 1 kit (48 extractions) | biochain |
Z1322015 | AnaPrep Total RNA Extraction Kit | 1 kit | biochain |
Z1322017 | AnaPrep Total RNA Extraction Kit with DNase-Treatment | 1 kit | biochain |
Z1322003 | AnaPrep Viral Nucleic Acid Extraction Kit (48) For extracting viral nucleic acids from plasma | 1 kit (48 extractions) | biochain |
Z1322011 | AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit A | 1 kit | biochain |
Z1322012 | AnaPrep Viral/Pathogen Nucleic Acids Extraction Kit B | 1 kit | biochain |
T2235090-SB | CancerSeq™ Paraffin Tissue Tumor Block: Colon | 1 Block | biochain |
T2235152-SB | CancerSeq™ Paraffin Tissue Tumor Block: Lung | 1 Block | biochain |
T2235086-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Breast | 5 Slides | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235152-SC | CancerSeq™ Plus Paraffin Tissue Tumor Section: Lung | 5 Curls | biochain |
T2235086-SB | CancerSeq™ Paraffin Tissue Tumor Block: Breast | 1 Block | biochain |
T2235218A-SB | CancerSeq™ Paraffin Tissue Tumor Block: Skin Melanoma | 1 Block | biochain |
T2235090-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Colon | 5 Slides | biochain |
T2235152-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Lung | 5 Slides | biochain |
T2235218A-ST | CancerSeq™ Paraffin Tissue Tumor Slides: Skin Melanoma | 5 Slides | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235086-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Breast | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
T2235248-SC | CancerSeq™ Plus Paraffin Tissue Tumor Sections: Stomach | 5 Curls | biochain |
Z1510001 | SeqPure PCR Purification Kit (5 ml) | 5 ml | biochain |
Z1510002 | SeqPure PCR Purification Kit (50 ml) | 50 ml | biochain |
Z1510003 | SeqPure PCR Purification Kit (500 ml) | 500 ml | biochain |
L1271010 | T4 RNA Ligase 1 | 1000 Units | biochain |
L1271050 | T4 RNA Ligase 1 | 5000 Units | biochain |
L1271500 | T4 RNA Ligase 1 | 50000 Units | biochain |
L1270010 | T4 RNA Ligase 2, truncated | 1000 Units | biochain |
L1270500 | T4 RNA Ligase 2, truncated | 50000 Units | biochain |
KS072012-I | Aligner 1-12 | 12 | biochain |
KS072012-II | Aligner 13-24 | 1 Kit | biochain |
KS072012-III | Aligner 25-36 | 1 Kit | biochain |
KS072012-IV | Aligner 37-48 | 1 Kit | biochain |
KS071012-12 | Gel Breaker Tubes | 12 | biochain |
KS071012-11 | Gel Cutter | 12 | biochain |
KS071012-13 | Gel Filter | 12 | biochain |
KS073012-I | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 1-12 | 1 Kit | biochain |
KS073012-II | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 13-24 | 1 Kit | biochain |
KS073012-III | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 25-36 | 1 Kit | biochain |
KS073012-IV | RapidSeq High Yield Directional mRNA Sample Prep Kit – With Aligner 37-48 | 1 Kit | biochain |
KS073012 | RapidSeq High Yield Directional mRNA Sample Prep Kit – Without Aligner | 1 Kit | biochain |
KS073012-T | RapidSeq High Yield Directional mRNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners | 1 Kit | biochain |
KS074012-I | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 1-12 | 1 Kit | biochain |
KS074012-II | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 13-24 | 1 Kit | biochain |
KS074012-III | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 25-36 | 1 Kit | biochain |
KS074012-IV | RapidSeq High Yield Small RNA Sample Prep Kit – With Aligner 37-48 | 1 Kit | biochain |
KS074012 | RapidSeq High Yield Small RNA Sample Prep Kit – Without Aligners | 1 Kit | biochain |
KS074012-T | RapidSeq High Yield Small RNA Sample Prep Kit, Trial version enough for 2 rxn. Customer select aligners | 1 Kit | biochain |
K5017100 | Blood and Serum DNA Isolation Kit | 1 kit | biochain |
K5011610 | cfPure® Cell Free DNA Extraction Kit (100 ml) | 1 Kit (100 ml) | biochain |
K5011625 | cfPure® Cell Free DNA Extraction Kit (250 ml) | 1 Kit (250 ml) | biochain |
K5011625MA | cfPure® MAX Cell-Free DNA Extraction Kit | 1 Kit (250 ml) | biochain |
K5019100 | FFPE Tissue DNA Extraction Kit | 1 kit | biochain |
K5011350 | FFPE Tissue DNA Extraction Kit – Column | 1 kit | biochain |
K5011450 | FFPE Tissue DNA Extraction Kit — Magnetic Beads | 1 kit | biochain |
K5016005 | Genomic DNA Extraction Kit | 1 kit | biochain |
K5011050 | Saliva DNA Isolation Kit | 1 kit | biochain |
K5018100 | Serum DNA Isolation Kit | 1 kit | biochain |
K5011250 | Stool DNA Isolation Kit | 1 kit | biochain |
K5011150 | Urine DNA Isolation Kit | 1 kit | biochain |
Z5060009 | xMag-Streptavidin Microparticles | 1 ml | biochain |
T6234701-1 | FDA Standard Frozen Tissue Array – Human Adult Normal | 2 slides | biochain |
T6234701-2 | FDA Standard Frozen Tissue Array – Human Adult Normal | 4 slides | biochain |
T6334701-1 | FDA Standard Frozen Tissue Array – Mouse Normal | 2 slides | biochain |
T6334701-2 | FDA Standard Frozen Tissue Array – Mouse Normal | 4 slides | biochain |
T6434701-1 | FDA Standard Frozen Tissue Array – Rat Normal | 2 slides | biochain |
T6434701-2 | FDA Standard Frozen Tissue Array – Rat Normal | 4 slides | biochain |
T6235201 | Frozen Tissue Array – 24 Different Prostate Tumors and 4 Corresponding Normal Controls | 1 slide | biochain |
T6235086-1 | Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235086-5 | Frozen Tissue Array – 37 Different Breast Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235090-1 | Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235090-5 | Frozen Tissue Array – 37 Different Colon Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235142-1 | Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235142-5 | Frozen Tissue Array – 37 Different Kidney Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235183-1 | Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls | 1 slide | biochain |
T6235183-5 | Frozen Tissue Array – 37 Different Ovarian Tumors and 3 Corresponding Normal Controls | 5 slides | biochain |
T6235152-1 | Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls | 1 slide | biochain |
T6235152-5 | Frozen Tissue Array – 40 Different Lung Tumors and 5 Corresponding Normal Controls | 5 slides | biochain |
T6734700-G02-1 | Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6734700-G02-5 | Frozen Tissue Array – Dog Female Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6734700-G01-1 | Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6734700-G01-5 | Frozen Tissue Array – Dog Male Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6234700-1 | Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6234700-5 | Frozen Tissue Array – Human Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6235700-1 | Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6235700-5 | Frozen Tissue Array – Human Tumor and Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6834700-G02-1 | Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6834700-G02-5 | Frozen Tissue Array – Rabbit Female Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6834700-G01-1 | Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I | 1 slide | biochain |
T6834700-G01-5 | Frozen Tissue Array – Rabbit Male Adult Normal Tissue, Multi-tissue I | 5 slides | biochain |
T6134035-1 | Multiple Species Frozen Tissue Array – Brain | 1 slide | biochain |
T6134035-5 | Multiple Species Frozen Tissue Array – Brain | 5 slides | biochain |
T6134149-1 | Multiple Species Frozen Tissue Array – Liver | 1 slide | biochain |
T6134149-5 | Multiple Species Frozen Tissue Array – Liver | 5 slides | biochain |
上海起發實驗試劑有限公司是實驗試劑一站式采購服務商
1：強大的進口輻射能力，血清、抗體、耗材、大部分限制進口品等。
2：產品種類齊全，經營超過700多個品牌，基本涵蓋所有生物實驗試劑耗材。
3：提供加急服務，貨品一般1-2周到貨。
4：富有競爭力的價格優勢，絕大部分價格有優勢。
5：多年積累良好的信譽，大部分客戶提供貨到付款服務。客戶包括清華、北大、交大、復旦、中山等100多所高校，ROCHE，阿斯利康、國藥、fisher等藥企。
6：我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech；sysy,TriLink BioTechnologies,Inc；worthington-biochem,zyagen等幾十家國外公司授權代理。
7：我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批發，歡迎合作。
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地址：上海浦東川沙鎮川沙路6619號上海起發實驗試劑有限公司
郵箱：xs1@78bio.com
傳真：021-50724961